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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
23/09/2019 |
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Actualizado : |
23/09/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
COUTO, D.; STRANSFELD, L.; ARRUABARRENA, A.; ZIPFEL, C.; LOZANO-DURÁN, R. |
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Afiliación : |
DANIEL COUTO, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; LENA STRANSFELD, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CYRIL ZIPFEL, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; ROSA LOZANO-DURÁN, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; Shanghai Institutes of Biological Sciences, Shanghai Center for Plant Stress Biology (PSC), Chinese Academy of Sciences, China. |
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Título : |
Broad application of a simple and affordable protocol for isolating plant RNA. (Short Report). |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
BMC Research Notes, 16 April 2015, Volume 8, Issue 1, Article number 154. OPEN ACCESS. |
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ISSN : |
1756-0500 |
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DOI : |
10.1186/s13104-015-1119-7 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published on: 16 April 2015. |
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Contenido : |
Abstract View references (4)
Background: Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner. Findings: We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents. Conclusions: Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide.
© 2015 Couto et al.; licensee BioMed Central. |
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Palabras claves : |
Affordable; Arabidopsis; Protocol; RNA; Tomato; Wheat. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13321/1/Couto-et-al.-2015.-BMC-Research-Notes.pdf
https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-015-1119-7
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Marc : |
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008 2015 bl uuuu u00u1 u #d
022 $a1756-0500
024 7 $a10.1186/s13104-015-1119-7$2DOI
100 1 $aCOUTO, D.
245 $aBroad application of a simple and affordable protocol for isolating plant RNA. (Short Report).$h[electronic resource]
260 $c2015
500 $aArticle history: Published on: 16 April 2015.
520 $aAbstract View references (4) Background: Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner. Findings: We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents. Conclusions: Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide. © 2015 Couto et al.; licensee BioMed Central.
653 $aAffordable
653 $aArabidopsis
653 $aProtocol
653 $aRNA
653 $aTomato
653 $aWheat
700 1 $aSTRANSFELD, L.
700 1 $aARRUABARRENA, A.
700 1 $aZIPFEL, C.
700 1 $aLOZANO-DURÁN, R.
773 $tBMC Research Notes, 16 April 2015, Volume 8, Issue 1, Article number 154. OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
25/06/2019 |
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Tipo de producción científica : |
Documentos |
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Autor : |
MONTOSSI, F.; DE MATTOS, D.; SAN JULIÁN, R.; DE LOS CAMPOS, G.; MEDEROS, A.; FRUGONI, J.C.; ZAMIT, W.; LEVRATTO, J.; MARTÍNEZ, H.; DE BARBIERI, I.; GRATTAROLA, M.; PÉREZ JONES, J. |
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Afiliación : |
FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DANIEL DE MATTOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ROBERTO SAN JULIAN SANCHEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUSTAVO DE LOS CAMPOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; AMERICA ESTHER MEDEROS SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JULIO CESAR FRUGONI SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; WILFREDO SHAMIL ZAMIT DUARTE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN CARLOS LEVRATTO CORTES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIS IGNACIO DE BARBIERI ETCHEBERRY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SUL (Secretariado Uruguayo de la Lana). |
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Título : |
Núcleo fundacional del Proyecto Merino Fino del Uruguay. |
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Fecha de publicación : |
2001 |
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Fuente / Imprenta : |
ln: INIA Tacuarembó; Unidad Experimental Glencoe. Producción animal y pasturas en Basalto. Día de campo, 15 noviembre, Paysandú, 2001. Tacuarembó (Uruguay): INIA, 2001. |
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Páginas : |
p. 12-14 |
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Serie : |
(INIA Serie Actividades de Difusión ; 271) |
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Idioma : |
Español |
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Contenido : |
Objetivos: Desarrollar una alternativa de producción ovina que por medio de su difusión y posterior adopción, permita mejorar la sustentabilidad socioeconómica de los productores de lana de las regiones de Basalto y Cristalino, considerando las demandas actuales y futuras de la Cadena Agroindustrial de lana del país y de los mercados consumidores. |
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Thesagro : |
OVINOS; RAZAS (ANIMALES); SUELO BASALTICO; URUGUAY. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/9721/1/SAD-271p12-14.pdf
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Marc : |
LEADER 01339naa a2200325 a 4500
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100 1 $aMONTOSSI, F.
245 $aNúcleo fundacional del Proyecto Merino Fino del Uruguay.
260 $c2001
300 $ap. 12-14
490 $a(INIA Serie Actividades de Difusión ; 271)
520 $aObjetivos: Desarrollar una alternativa de producción ovina que por medio de su difusión y posterior adopción, permita mejorar la sustentabilidad socioeconómica de los productores de lana de las regiones de Basalto y Cristalino, considerando las demandas actuales y futuras de la Cadena Agroindustrial de lana del país y de los mercados consumidores.
650 $aOVINOS
650 $aRAZAS (ANIMALES)
650 $aSUELO BASALTICO
650 $aURUGUAY
700 1 $aDE MATTOS, D.
700 1 $aSAN JULIÁN, R.
700 1 $aDE LOS CAMPOS, G.
700 1 $aMEDEROS, A.
700 1 $aFRUGONI, J.C.
700 1 $aZAMIT, W.
700 1 $aLEVRATTO, J.
700 1 $aMARTÍNEZ, H.
700 1 $aDE BARBIERI, I.
700 1 $aGRATTAROLA, M.
700 1 $aPÉREZ JONES, J.
773 $tln: INIA Tacuarembó; Unidad Experimental Glencoe. Producción animal y pasturas en Basalto. Día de campo, 15 noviembre, Paysandú, 2001. Tacuarembó (Uruguay): INIA, 2001.
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INIA Tacuarembó (TBO) |
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